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KMID : 1134820120410050584
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Journal of the Korean Society of Food Science and Nutrition
2012 Volume.41 No. 5 p.584 ~ p.590
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Induction of Apoptosis in Human Cancer Cells with Compositae Extracts
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An In-Jung
Kwon Jung-Ki
Lee Jin-Seok
Park Ha-Seung
Kim Dong-Chan
Choi Byung-Jun
Lee Kyu-Min
Park Yong-Jin
Jung Ji-Youn
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Abstract
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Dried Compositae flowers have traditionally been used for the treatment of anti-inflammatory and anti-oxidative stress in Korea. This paper investigates the effects of Compositae extracts on the inhibition of proliferation and apoptosis of human gastric cancer AGS cells, human breast cancer MDA-MB-231 cells, and SK-BR-3 cells. The proliferation of AGS cells, MDA-MB-231 cells, and SK-BR-3 cells were determined by MTT assay. Several Compositae extracts inhibited proliferation of AGS cells, MDA-MB-231 cells, and SK-BR-3 cells in a dose-dependent manner. To assess the apoptosis of Compositae extracts, the nuclei of MDA-MB-231 cells were stained with DAPI. The presence of chromatin condensation in the Compositae extract-treated cells was detected on a fluorescent microscope (×200). We conducted Western blot analysis of changes in Bcl-2, Bax, and p53 protein expression levels. Apoptosis by Chrysanthemum zawadskii subsp. coreanum, Chrysanthemum zawadskii var. tenuisectum and Rudbeckia laciniata var. hortensis treatment created a decrease in Bcl-2 expression, whereas the expression of Bax and p53 were increased. These results indicate that Chrysanthemum zawadskii subsp. coreanum, Chrysanthemum zawadskii var. tenuisectum and Rudbeckia laciniata var. hortensis inhibit breast cancer cell growth through the induction of apoptosis.
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KEYWORD
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Compositae, anticancer, breast cancer, apoptosis
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